Protein deficiency leads to faster fat burning in mice

Protein deficiency leads to faster fat burning in mice, study
shows

COLUMBUS, Ohio Researchers have developed a new, lean mouse with characteristics
suggesting that someday, using medication to manipulate a specific protein in humans could emerge
as a strategy to treat obesity and disorders associated with excess weight, such as diabetes and
metabolic syndrome.

To create the hybrid, scientists crossed mice deficient in protein kinase C beta (PKCB) with the C57
black mouse, a common animal used in research for studying diabetes and obesity.

"These animals can eat more than normal. And they have less fat than normal. That's a dream come
true if it can be extended to human beings," said Kamal Mehta, senior author of the study and a
professor of molecular and cellular biochemistry at Ohio State University.

He noted, however, that an appropriate therapy for humans would take years to develop.

At first glance, compared to mice with no deficiency, these new hybrid mice were smaller and
leaner. And when the researchers looked under their skin, they saw the mice had less fat distribution
in the skin itself and less fat tissue overall. They also had less fat in their livers and muscles. The fat
cells they did have were smaller than fat cells in other mice.

And despite the propensity for obesity from their original genes, the new mice lost weight while
eating up to 30 percent more food than other mice. This means their lower weight was not caused
by less eating, suggesting the protein deficiency corrected for the obesity tendencies by increasing
the hybrids' ability to burn fat, said Mehta, an investigator in Ohio State's Davis Heart and Lung
Research Institute.

The research is published in a recent issue of the Journal of Biological Chemistry.

Based on his previous research on the role of PKCB in metabolism, Mehta expected a deficiency of
the protein to affect how the body processes triglycerides, or fat stored in body tissue.

"The bottom line is we were the first to show that this deficiency leads to a lean animal. The next
question is why," Mehta said. "In order to answer why, we need to know which genes are changed
in these knockout animals."

The most prominent effect the scientists have been able to identify so far relates to the
mitochondria, the principal energy source of cells. Mehta said the new hybrid mice have more
mitochondria within their cells than do normal mice, and that the added energy source allows them
to convert fatty acids into energy.

"We have shown to some extent that there is increased fatty acid oxidation.

We found that they use more oxygen, so that means they are using this oxygen to metabolize fat,
convert it into carbon dioxide and expel it when they breathe," said Madhu Mehta, a clinical
consultant and co-author on the study and assistant professor of internal medicine at Ohio State.

The research group is testing this finding with an additional experiment, introducing the PKCB

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